Store in a dry place away from light, tighten the cap immediately after use.
Triple Sugar Iron Agar II | Application | Used to identify the biochemical reactions of enteric bacteria fermenting sucrose, lactose, glucose and producing hydrogen sulfide. |
| Principle | Peptones, beef leaching powder provide nitrogen source, vitamins, minerals; lactose, glucose, sucrose are fermentable sugars, their acid production is measured by phenol sulfonphthalein indicator, yellow in acidity, red in alkalinity; sodium thiosulfate can be reduced to hydrogen sulfide by some bacteria, and with iron salts in ferrous sulfate to produce black iron sulfide; sodium chloride maintains balanced osmolality; agar is the coagulant for the culture medium. |
| Formulation (/L) |
Peptone 20.0 g Beef leaching powder 5.0 g Lactose 10.0 g Sucrose 10.0 g Glucose 1.0 g Sodium chloride 5.0 g Ferrous sulfate 0.2g Sodium thiosulfate 0.2g Agar 12.0 g Phenolphthalein 0.025 g |
| Directions | Weigh 63.4g of this product, add distilled water or deionized water 1 L, heating and stirring to dissolve and boil until completely dissolved, divided into test tubes (loading volume should be more). Put the test tube into the sterilizer, 121°C autoclave sterilization 15min, take out, made of high-level slant, standby. |
| Shelf Life | 3 years |
Store in a dry place away from light, tighten the cap immediately after use.
CD BioSciences is a well-known professional company in the industry. We provide a wide range of bio-environmental products, including bio-based materials, biomass fuels, green building materials, bio-environmental enzymes, bio-environmental microorganisms, etc. Please feel free to explore.
Our products and services are for research use only and cannot be used for any clinical purposes.
