Resequencing

In the era of rapid biodiversity loss and environmental degradation, accurate species identification and understanding genetic diversity are paramount for effective conservation efforts. Traditional methods of species identification based on morphological characteristics can be time-consuming, subjective, and often inaccurate, especially for cryptic species or when dealing with degraded samples. Advances in DNA sequencing technologies have revolutionized the field of species identification, offering powerful tools for biodiversity assessment, conservation monitoring, and ecological research.

Resequencing, also known as whole-genome resequencing, is a cutting-edge genomic technique that involves sequencing the entire DNA of an organism and comparing it to a known reference genome. This process allows for the identification of genetic variations, including single nucleotide polymorphisms (SNPs), insertions and deletions (InDels), structural variations (SVs), and copy number variations (CNVs). These variations provide a unique genetic fingerprint that can be used for accurate species identification, even in cases where traditional methods fail.

Fig 1. Workflow for mediator based resequencing (Omri W., 2010)

Service Overview

• Our company offers comprehensive resequencing services for a wide range of applications, including:
  Species identification: Accurate and reliable identification of species from various sample types, including tissue, hair, feathers, and environmental DNA (eDNA).
• Biodiversity assessment: Comprehensive analysis of species diversity and community composition in different ecosystems.
• Conservation genetics: Assessment of genetic diversity and population structure for endangered species and conservation management.

• Evolutionary studies: Investigation of evolutionary relationships and phylogenetic patterns among species.
• Environmental monitoring: Detection and monitoring of invasive species, disease outbreaks, and environmental pollution.

Our resequencing services leverage state-of-the-art sequencing technologies and bioinformatics pipelines to deliver high-quality data and comprehensive analysis reports. Our team of experienced scientists and bioinformaticians are committed to providing our clients with accurate, reliable, and timely results.

Technological Principles

• DNA extraction: High-quality DNA is extracted from the sample using appropriate methods.
• Library preparation: The extracted DNA is fragmented and prepared for sequencing.
• Sequencing: The DNA library is sequenced using next-generation sequencing (NGS) technologies, generating millions of short DNA reads.
• Alignment and variant calling: The sequenced reads are aligned to a reference genome, and genetic variations are identified.
• Data analysis: The identified variations are analyzed to determine species identity, genetic diversity, and other relevant information..


Technical Features

• High accuracy: Resequencing provides accurate and reliable species identification, even for closely related species or degraded samples.
• High throughput: NGS technologies allow for the simultaneous sequencing of multiple samples, increasing efficiency and reducing costs.
• Comprehensive data: Resequencing provides a wealth of genetic information, including SNPs, InDels, SVs, and CNVs.
• Versatility: Resequencing can be applied to a wide range of sample types and species.

Technical Classifications

Resequencing can be classified into different types based on the sequencing depth and coverage:

• Low-coverage resequencing: This involves sequencing the genome at a low depth, typically 1-5X coverage. It is suitable for species identification and basic genetic diversity analysis.
• Medium-coverage resequencing: This involves sequencing the genome at a medium depth, typically 5-30X coverage. It is suitable for more detailed genetic analysis, including population genetics and evolutionary studies.
• High-coverage resequencing: This involves sequencing the genome at a high depth, typically 30X or higher coverage. It is suitable for comprehensive genomic analysis, including the identification of rare variants and structural variations.


Applications

Resequencing has a wide range of applications in various fields, including:

• Conservation biology: Resequencing can be used to assess genetic diversity, population structure, and connectivity of endangered species, providing valuable information for conservation management.
• Ecology: Resequencing can be used to study species interactions, community dynamics, and ecosystem functioning.
• Evolutionary biology: Resequencing can be used to investigate evolutionary relationships, phylogenetic patterns, and adaptation mechanisms.
• Agriculture: Resequencing can be used for crop improvement, livestock breeding, and disease resistance.
• Forensic science: Resequencing can be used for human identification, criminal investigations, and paternity testing.

Environmental Benefits


• Non-invasive sampling: Resequencing can be performed on non-invasive samples, such as eDNA from water or soil, reducing the need for capturing or handling organisms.
• Early detection of invasive species: Resequencing can be used to detect invasive species at low abundance, enabling early intervention and preventing ecological damage.
• Monitoring of endangered species: Resequencing can be used to monitor the genetic health and population trends of endangered species, aiding in conservation efforts.
• Assessment of environmental pollution: Resequencing can be used to detect and monitor genetic changes in organisms caused by environmental pollution, providing valuable information for environmental management.

Our Services

At our bio-environmental company, we specialize in providing comprehensive re-sequencing services that leverage the latest next-generation sequencing (NGS) technologies to deliver insights into genetic variations within species. Here's an overview of the services we offer:


• De novo Sequencing and Re-sequencing of Genomes: We perform both de novo sequencing for novel species and re-sequencing of known genomes to identify genetic variations such as SNPs, InDels, and SVs.
• Transcriptome Sequencing and Analysis: Our services include transcriptome sequencing for both eukaryotic and prokaryotic organisms, providing a comprehensive view of gene expression profiles.
• Metagenome Sequencing and Analysis: We offer metagenomic sequencing to analyze genetic material from environmental samples, providing insights into microbial communities.
• Targeted Re-sequencing: We specialize in targeted re-sequencing of specific genomic regions of interest, such as exomes, to identify functional variants associated with specific traits or diseases.
• Unique Multiplexing System: Our advanced multiplexing system allows for the processing of a high number of samples, ensuring efficient and cost-effective analysis.
• Extensive Bioinformatics Services: We provide robust bioinformatics support, including alignment, variant calling, and annotation to ensure accurate interpretation of sequencing data.

Distinctive Service Features

• State-of-the-Art Sequencing Platforms: We utilize cutting-edge sequencing platforms like Illumina's high-throughput sequencers, which are known for their accuracy and reliability.
• Customized Analysis Strategies: We offer tailored analysis strategies based on the specific needs of different sequencing projects, including the selection of reference genome versions and alignment algorithms.
• Comprehensive Database Integration: Our services are backed by continuous updates and integration of multiple databases, ensuring accurate gene information and annotations.
• Powerful Omics Integration Analysis: We integrate genome resequencing with other omics technologies, such as transcriptome and methylation sequencing, to provide a holistic view of genetic variations.

Contact Us



By choosing our re-sequencing services, you are partnering with a company that is at the forefront of genomics technology, committed to delivering high-quality data, and providing the support needed to make sense of complex genetic information. Our goal is to empower researchers and organizations with the tools and knowledge to advance their understanding of genetic diversity and make informed decisions in their respective fields. Contact us today to learn more about how our services can support your research or breeding program.

How to Place an Order



Reference

1. Omri W., Mally D., et al. "Mutation Detection with Next-Generation Resequencing through a Mediator Genome" PLOS ONE 2010, 5(12):15628.

Our products and services are for research use only and cannot be used for any clinical purposes.