Recombinant Kex2 Protease Re15 | Product Advantage |
Non-active: recombinant production, no exogenous virus contamination, the production process does not use any animal source raw materials. Stable quality: mass production can ensure stable and continuous batch production; there is no difference between product batches and stable quality. High purity: the specific activity is high; the host protein residue is less than the limit requirement of biological products. Freeze-dried powder: easy to store and transport. Compliance with regulatory requirements: the production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines. Quality documents complete: according to customer requirements, relevant regulatory supporting documents can be provided. |
| Enzymatic Properties | Recombinant Kex2 protease is produced by Pichia pastoris expression, and has the same enzyme specificity as the natural Saccharomyces cerevisiae Kex2 enzyme. |
| Basic Characteristics | Recombinant Kex2 protease also known as recombinant double base endonuclease, YSCF protease, Kex2 protease, in yeast, the Kex2 protease is responsible for processing the precursors of killer toxin and α-factor. Kex2 protease activity is not inhibited by conventional serine protease inhibitors such as aprotinin, PMSF, TPCK. The optimum pH was 9.0, and the stable storage pH was 5.0-6.0. |
| Method of Use |
Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2 or HEPES, 5 mM Ca2. If it is not used immediately after dissolution, it is recommended to use 20 mM (pH 5.2) NaAc-HAc, 2 mM Ca2 the freeze-dried powder is dissolved in the buffer solution, and the final concentration of the dissolved enzyme is about 1-10 mg/ml, which is stored below -20°C after being divided as required. Reaction with pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2 or HEPES, 5 mM Ca2 as reaction diluent. Note: the optimum reaction pH of the enzyme was 9.0 and the stable pH was 5.0-6.0. |
| Sizes | 1mg; 5mg; 10mg; 100mg; 1g |
| Enzyme Aite | Kex2 protease is a calcium-dependent proteolytic enzyme that can specifically recognize and cleave the sequences Arg-Arg (arginine, R) and Lys-Arg (lysine, K). Unlike trypsin, Kex2 does not recognize and cleave the carboxy-terminal peptide bond of a single basic amino acid, either arginine or lysine. |
| Appearance | White, almost white powder |
| Specific Activity | ≥ 10.0 units/mg pro. |
| Protein Electrophoresis | Single Master Strip |
| Molecular Weight (Protein Electrophoresis) | 67.0±6.7 kD |
| Remarks | In a 3ml reaction system at 25°C, 50 mM Tris-HCl, 2 mM CaCl2, pH8.0, the amount of enzyme that catalyzes the Boc-QRR-pNA release of 1 μmol of p-nitroaniline (4-nitroaniline) per minute is defined as an enzyme activity unit. |
| Storage | Freeze-dried powder storage stability: 2-8°C, dark, moisture-proof preservation. Dissolution preservation buffer (20 mM NaAc-HAc, pH 5.2, 2 mM Ca2), stored below -20°C after dissolution, stable for 12 months. |
| Transportation | Transport stability: blue ice insulation transport, stable activity. |
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