Recombinant Aprotinin | Introduction | Aprotinin is a competitive inhibitor of serine proteases and can inhibit the activities of trypsin, chymotrypsin, and kininogenase. It forms a stable complex with the protease, thereby blocking the active site of the enzyme. Enzymes derived from aprotinin share similar characteristics with animal-derived peptide enzymes and can be substituted for them. Aprotinin of Animal Origin is utilized in various biotechnological processes, including recombinant protein production and cell culture. |
| Product Advantage |
Free of animate origin: recombinant production, no exogenous virus contamination, the production process does not use any animal source raw materials. Quality: mass production,can ensure stable continuous batch production; Productsthere is no difference between batches,quality. Purity: host protein residue is less than the biological product limit requirement. Freeze-dried powder: easy to store and transport. Compliance with regulatory requirements: The production equipment and production environment comply with the relevant regulatory requirements, and the production process is fully compliant. follow NSF ISO 9001: 2015 quality system and compliance GMP guiding principles. Quality documents complete: According to customer needs, can provide relevant regulatory support documents. |
| Mechanism of Action | Aprotinin forms a stable complex with a protease to block the active site of the enzyme. Most of the peptideenzyme-protease complexin pH <3.0 conditions. Aprotinin is effectively equimolar to protease. |
| Enzymatic Properties | Recombinant aprotinin has the same enzymatic properties as animal-derived aprotinin and can be used instead of animal-derived aprotinin in various biotechnological processes. |
| Basic Characteristics | Recombinant aprotinin is also knownrecombinant trypsin inhibitor,peptideenzyme (Cowlung), anti-proteasepeptide (Cowlung), protease inhibitor, aprotinin is a competitive inhibitor of serine proteases and can inhibit the activities of trypsin, chymotrypsin, and kininogenase. |
| Method of Use |
Aprotinin and protease are equimolar effective binding, Recommended Combination pH >6.0, in pH <3.0 conditions. No. combination. Can directly make used 0.9% NaCl dissolution, after dissolution available-20°C storage. |
| Sizes | 1mg; 10mg; 100mg; 1g; 10g; 50g; 100g |
| Amino Acid Sequence | Recombinant aprotinin was expressed in recombinant E. Coli, multiple column, is identical to that of aprotinin from bovine lung. |
| Appearance | White, almost white, yellow-like powder |
| Specific Activity | ≥ 3.0 EPU/mg pro. |
| Purity | Purity (HPLC): ≥ 95% |
| Absorbance (277nm) | ≤ 0.8 (3 EPU/ml aqueous solution) |
| Solubility (5 mg/ml in 0.9% NaCl) | Soluble, clear or slightly turbid |
| Protein Content (%) | ≥ 60% |
| Storage | Storage stability: Recombinant aprotinin freeze-dried powder storage in 2-8°C, 24months stable. |
| Transportation | Transport stability: blue ice insulation transport, stable activity. |
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