In the realm of molecular biology, the advent of real-time fluorescent quantitative PCR (qPCR) has revolutionized the way we analyze and quantify nucleic acids. This powerful technique has found widespread applications across various fields, including diagnostics, research, and environmental monitoring. At CD BioSciences, a leading bioenvironmental company, we leverage the cutting-edge technology of qPCR to provide a comprehensive suite of services aimed at delivering accurate, reliable, and timely results to our clients.
Fig 1. Real-time Fluorescent Quantitative PCR (Wang S., 2014)
Service Overview
Our qPCR services are designed to cater to a diverse range of needs, from SNP genotyping and gene expression analysis to pathogen detection and environmental monitoring. We specialize in utilizing qPCR to identify Single Nucleotide Polymorphisms (SNPs), which are minute variations in DNA sequences that can have profound implications for disease susceptibility, drug responses, and other phenotypic traits.
Technological Principles
qPCR is a sophisticated technique that allows for the real-time monitoring of DNA amplification during the PCR process. It works by utilizing fluorescent dyes or probes that bind to the DNA molecules being amplified. As the DNA amplifies, the fluorescence signal increases proportionally, enabling precise quantification of the target DNA sequence.
Technical Features
- High Sensitivity: qPCR can detect even trace amounts of DNA, making it ideal for analyzing limited or degraded samples.
- High Specificity: The use of specific primers and probes ensures accurate targeting of the desired DNA sequence, minimizing non-specific amplification.
- High Throughput: qPCR can be performed in a high-throughput format, allowing for simultaneous analysis of multiple samples, saving time and resources.
- Real-time Monitoring: The ability to monitor DNA amplification in real-time provides immediate feedback on the progress of the reaction and enables precise quantification.
Types of HRM Analysis
qPCR can be broadly classified into two main categories:
- Dye-based qPCR: This method utilizes fluorescent dyes that bind to double-stranded DNA, providing a general measure of DNA amplification.
- Probe-based qPCR: This approach employs fluorescently labeled probes that specifically bind to the target DNA sequence, offering greater specificity and accuracy.
Applications of HRM in Environmental Science
The versatility of qPCR makes it applicable across a wide range of fields:
- SNP Genotyping: Identifying SNPs associated with diseases, drug responses, and other phenotypic traits.
- Gene Expression Analysis: Quantifying gene expression levels to study cellular processes, disease progression, and drug effects.
- Pathogen Detection: Detecting and quantifying pathogenic microorganisms in various samples, including food, water, and clinical specimens.
- Environmental Monitoring: Assessing microbial communities in environmental samples, aiding in bioremediation and pollution control efforts.
Environmental Benefits
qPCR services are designed to minimize environmental impact. It utilize environmentally friendly reagents and consumables whenever possible and adhere to strict waste disposal protocols. By adopting sustainable practices, it contribute to a healthier planet while delivering high-quality services.
Our Services
At our bio-environmental company, we specialize in providing cutting-edge real-time fluorescent quantitative PCR (qPCR) services for SNP identification, enabling precise species identification at the molecular marker level. Our services are designed to meet the demands of modern molecular biology and genetics research. Here's an overview of the services we offer and the advantages of choosing us for your qPCR needs.
- Gene Copy Number Detection: We offer qPCR services for determining the copy number of genes at the genomic DNA level, which is crucial for genetic research and diagnostics.
- Gene Expression Analysis: Our qPCR services include the analysis of mRNA levels, providing insights into gene expression patterns.
- miRNA Level Detection: We also provide qPCR services for detecting miRNA levels, which are important for understanding post-transcriptional gene regulation.
- SNP Genotyping Analysis: Utilizing qPCR, we can accurately identify single-nucleotide polymorphisms (SNPs), which are vital for genetic studies and molecular diagnostics.
- High-Throughput SNP Detection: We employ multiplex PCR and high-throughput sequencing to detect a large number of SNPs simultaneously, offering a cost-effective and efficient solution for large-scale genetic studies.
s, contributing to the development of personalized medicine.
- Custom qPCR Assays: We design and optimize custom qPCR assays tailored to your specific research needs, including the development of specific primers and probes.
- Data Analysis and Reporting: We provide comprehensive data analysis and reporting services to help you interpret your qPCR results effectively.
Distinctive Service Features
- High Sensitivity and Specificity: Our qPCR services are known for their high sensitivity and specificity, ensuring accurate quantification of target nucleic acids.
- Rapid Results: qPCR allows for rapid analysis, providing results in real-time, which is faster than traditional PCR methods.
- Ease of Use and Automation: Our qPCR services are designed with user-friendliness in mind, with automated workflows to minimize operator error and increase efficiency.
- Comprehensive Support: We offer complete support, from experimental design to data analysis, ensuring that your project is in expert hands from start to finish.
Contact Us
By choosing our qPCR services, you are partnering with a company that is committed to providing the highest quality molecular marker analysis for SNP identification and species identification. Contact us today to learn more about how our services can support your research.
How to Place an Order
Reference
- Wang S. "Real Time Polymerase Chain Reaction" Biomarkers in Toxicology 2014, 18(7):3123.
Our products and services are for research use only and cannot be used for any clinical purposes.
Real-time Fluorescent Quantitative PCR (qPCR)
