Pullulanase 
Catalog Number IE-1136
Physical Form Liquid
Color Brown Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.
Declared Activity 2,000 u/ml
| Benefits |
• Increases fermentable carbohydrates • Provides a higher level of attenuation with low impact on yeast vitality • Produces highly attenuated beer in a cost-effective manner • Normally inactivated at beer pasteurization |
| Dosage | The recommended dosage is 0.4-0.8 L of the enzyme preparation per ton of total raw materials. Pullulanase can reduce the content of amylopectin to produce dry beer. However, the optimal dosage depends on the specific process parameters and should be determined by testing different dosages at the brewery. |
| Activity Temperature | 40°C-65°C |
| Optimum Temperature | 50°C-60°C |
| Activity pH | 3.5-6.0 |
| Optimum pH | 4.0-5.0 |
| Production Organism | Bacillus subtilis |
| Production Method | Produced by submerged fermentation, separation and extraction technology. |
| Definition of Unit | 1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1 µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH 5.8, 50°C. |
| Declared Activity | 2,000 u/ml |
| Physical Form | Liquid |
| Color |
Brown Color may vary from batch to batch. Color intensity is not an indication of enzyme activity. |
| Odour | Normal microbial fermentation odour. |
| Lead | ≤5.0 mg/kg |
| Arsenic | ≤3.0 mg/kg |
| Total Viable Count | ≤50000 CFU/ml |
| Coliform Bacteria | ≤30 CFU/ml |
| Escherichia Coli | <10 CFU/ml, ≤3.0 MPN/ml |
| Salmonella/25g | Not Detected |
| Enzyme Activity | ≥ 2000 u/ml |
| Safe Handling Precautions | Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor. |
| Package | 25 kgs/drum, 1,125 kgs/drum |
| Storage | This product should be stored in a cool and dry place in sealed container, avoiding insolation, high temperature and damp. The product has been formulated for optimal stability. Extended storage or adverse conditions such as higher temperature or higher humidity may lead to a higher dosage requirement. |
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