Phenol Red Broth Base w/ Meat Extract

Phenol Red Broth Base w/ Meat Extract
Phenol Red Broth Base w/ Meat Extract

Catalog Number FM-2271

Product Name Phenol Red Broth Base w/ Meat Extract

Aspect Light yellow to pink powder

Product Information

Application The product is used for the study of carbohydrate fermentation.
Principle The complete medium can be augmented with carbohydrates to study fermentation reactions, indicated by medium color change to yellow due to acid production (phenol red as the pH indicator) and gas formation seen as bubbles in Durham’s tube.
pH 7.4±0.2
Colour and Clarity of Prepared Medium Red transparent solution, not turbid
Formulation (/L) Casein enzymic hydrolysate 10.0 g
Meat extract 1.0 g
Sodium chloride 5.0 g
Phenol red 0.018g
Directions Dissolve 16.02 g in 1000 mL of distilled water. Add the desired amount of test carbohydrate. Heat if necessary to dissolve the medium completely. Mix well and dispense in tubes containing inverted Durham tubes. Autoclave at 15 pounds pressure (121°C) for 15 minutes.

Storage

Keep in airtight containers below 30°C. Prepared media is kept at 2-8°C.

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