Keep in airtight containers below 30°C. Prepared media is kept at 2-8°C.
Catalog Number FM-2271
Product Name Phenol Red Broth Base w/ Meat Extract
Aspect Light yellow to pink powder
Application | The product is used for the study of carbohydrate fermentation. |
Principle | The complete medium can be augmented with carbohydrates to study fermentation reactions, indicated by medium color change to yellow due to acid production (phenol red as the pH indicator) and gas formation seen as bubbles in Durham’s tube. |
pH | 7.4±0.2 |
Colour and Clarity of Prepared Medium | Red transparent solution, not turbid |
Formulation (/L) |
Casein enzymic hydrolysate 10.0 g Meat extract 1.0 g Sodium chloride 5.0 g Phenol red 0.018g |
Directions | Dissolve 16.02 g in 1000 mL of distilled water. Add the desired amount of test carbohydrate. Heat if necessary to dissolve the medium completely. Mix well and dispense in tubes containing inverted Durham tubes. Autoclave at 15 pounds pressure (121°C) for 15 minutes. |
Keep in airtight containers below 30°C. Prepared media is kept at 2-8°C.
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