Phenol Red Broth Base
Phenol Red Broth Base

Catalog Number FM-2270

Product Name Phenol Red Broth Base

Aspect Light yellow to pink powder

Product Information

Application The product can be used to determine fermentation reactions of pure cultures of microorganisms.
Principle This medium differentiates various species and genera by acid and gas production.
pH 7.4±0.2
Colour and Clarity of Prepared Medium Red clear solution, no precipitate
Formulation (/L) Proteose peptone 10.0 g
peptone B # 1.0 g
Sodium chloride 5.0 g
Phenol red 0.018g
Directions Dissolve 16.02 g in 1000 ml of purified/distilled water and mix well. Heat if necessary to dissolve the medium completely. Mix well and dispense in fermentation tubes (tubes containing inverted Durham tubing). Autoclave at 15 pounds pressure (121°C) for 15 minutes. Add filter-sterilized or autoclaved carbohydrate solution to sterile basal medium.

Storage

Keep in airtight containers at 10-30°C. Prepared media is kept at 15-30°C.

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