Nucleoside Diphosphate Kinase for NTP generation 
Catalog Number IE-1388
Physical Form Liquid
Color Brown to dark brown
Dosage Parameters 0.1-1 kg/T of raw material
Source Microorganism
| Summary | The enzyme catalyzes, in a reversible manner, the terminal phosphate exchange between different nucleoside diphosphates (NDPs) and triphosphates (NTPs) to generate nucleoside triphosphates. |
| Description | Nucleoside Diphosphate Kinase is produced by submerged fermentation followed by separation, ultrafiltration and formulation. The enzyme catalyzes the transfer of a phosphate group between adenosine triphosphate (ATP) and nucleoside diphosphate (NDP) to maintain the NTP concentration in the cells of organisms. |
| Mechanism | Nucleoside diphosphate kinase is an enzyme that catalyzes, in a reversible manner, the terminal phosphate exchange between different nucleoside diphosphates (NDPs) and triphosphates (NTPs) to generate nucleoside triphosphates. Many NDPs serve as acceptors, while NTP serves as the donor of the phosphate group. The general reaction via a ping-pong mechanism is as follows: XDP + YTP ←→ XTP + YDP (where X and Y represent different nitrogenous bases). Nucleoside diphosphate kinase maintains the balance between different nucleoside triphosphate levels, for example, when guanosine triphosphate (GTP) generated in the citric acid cycle is converted to adenosine triphosphate (ATP). |
| Advantages | Catalyze the terminal phosphate exchange between different nucleoside diphosphates; maintain the NTP concentration in the cells of organisms. |
| Application | For NTP generation |
| Application Area |
Food Biosynthesis |
| Application Recommendation | The recommended dosage is 0.1-1 kg/T of raw material. The dosage has to be optimized based on real situation, the raw material specifications, product expectations and processing parameters. It is better to begin the test with the smallest quantity. |
| Dosage Parameters | 0.1-1 kg/T of raw material |
| Optimum Temp | 40-50°C |
| Effective Temp | 30-60°C |
| Optimal pH | 5.0-7.0 |
| Effective pH | 4.0-8.0 |
| Source | Microorganism |
| Physical Form | Liquid |
| Color | Brown to dark brown |
| Scope of Shipment | Worldwide |
| Package | 25 kg/jerry can (customizable) |
| Storage | Keep sealed in a dry place at below -8°C and avoid direct sunlight. |
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