Metatranscriptomic Sequencing

Service Overview

CD BioSciences offers cutting-edge metatranscriptomic sequencing services, empowering researchers and industries to delve into the intricacies of microbial communities. Our comprehensive services encompass every stage of the process, from sample preparation and sequencing to bioinformatic analysis and data interpretation. We are committed to delivering high-quality data and expert analysis, enabling our clients to make groundbreaking discoveries and advance their research and development endeavors.

• Sensitivity and Specificity: Metatranscriptomics offers high sensitivity, enabling the detection of even low-abundance transcripts. This allows for the identification of rare or transient microbial species that may play critical roles in the ecosystem. Additionally, the technique provides high specificity, accurately distinguishing between closely related species.
• Functional Insights: By analyzing the active genes, metatranscriptomics provides valuable insights into the functional capabilities of the microbial community. This information can be used to understand metabolic pathways, identify key enzymes, and assess the overall health and activity of the ecosystem.

Metatranscriptomic approaches can be broadly classified into two main categories:

• Targeted Metatranscriptomics: This approach focuses on the analysis of specific genes or gene families of interest, providing in-depth information about particular functions or pathways.
• Untargeted Metatranscriptomics: This approach takes a broader view, analyzing the entire transcriptome of the microbial community. This allows for the discovery of novel genes, unexpected pathways, and a more comprehensive understanding of the community's functional capabilities.

Biotechnology

• Enzyme Discovery: Metatranscriptomics can uncover novel enzymes with industrial applications, such as biocatalysts for biofuel production or enzymes for food processing.
• Drug Discovery: The technique can identify bioactive compounds produced by microbes, potentially leading to the development of new drugs and therapies.
• Agricultural Applications: Metatranscriptomics can be used to study plant-microbe interactions, optimize soil health, and improve crop yields.

• Reduced Environmental Impact: The technique requires smaller sample sizes compared to traditional methods, minimizing disturbance to ecosystems.
• Sustainable Solutions: Metatranscriptomics can identify microbes and pathways involved in sustainable processes, such as bioremediation and biofuel production.
• Environmental Monitoring: The technique can be used to monitor the health of ecosystems and detect early signs of environmental stress.

• Metatranscriptome Sequencing Services: We offer comprehensive services for extracting total RNA from environmental samples and conducting high-throughput sequencing to analyze the transcriptional activity of microbial communities.
• Species Identification and Activity Analysis: By aligning RNA sequence data, we can identify active species in the samples and analyze their expression levels and functions.
• Functional Gene Expression Analysis: We analyze the expression of functional genes within microbial communities to help clients understand the functional potential of these communities.
• Data Interpretation and Reporting: We provide detailed data analysis reports, including species composition, functional annotations, and differential expression analysis, to enable clients to gain a deep understanding of the microbial characteristics of their samples.
• Customized Analysis Solutions: Based on clients' research objectives, we design tailored analysis processes to meet specific scientific needs.
• Technical Support and Consultation: We offer professional technical support and consultation to assist clients with questions regarding project design and result interpretation.
• Sample Quality Control: We implement strict sample quality control procedures to ensure the accuracy and reliability of sequencing data.
• Multi-Omics Data Integration: We can integrate metatranscriptome data with other omics data (such as metagenomics, proteomics, and metabolomics) to provide a more comprehensive analysis of microbial communities.
• Long-Term Project Collaboration: We support long-term project collaborations, including continuous monitoring and tracking studies, to observe changes in microbial communities over time.
• Publication Support: We support clients in publishing their research findings in scientific journals by providing necessary data support and analysis method descriptions.