At CD BioSciences, we specialize in metabolites identification of biological compounds, providing cutting-edge technologies and comprehensive services to meet the needs of the biotechnological and environmental sectors. Our research-driven approach ensures accurate and insightful identification and analysis of metabolites, contributing significantly to advancements in both research and application.
Fig 1. Techniques for Detection and Extraction of Metabolites (Payal G,
et al., 2020)
Technical Principles
Metabolites are small molecules that are intermediates and products of metabolism. Identifying these compounds is crucial as they play essential roles in understanding biochemical processes, disease mechanisms, and potential biotechnological applications. Metabolite identification involves analyzing the chemical profiles of biological samples to detect and quantify these small molecules.
Technical Means
- Liquid Chromatography-Mass Spectrometry (LC-MS/MS): Combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry. Ideal for identifying and quantifying both polar and non-polar metabolites in complex mixtures.
- Gas Chromatography-Mass Spectrometry (GC-MS): A powerful tool for analyzing volatile and semi-volatile organic compounds. Provides high-throughput analysis and is especially useful for environmental sample analysis.
- Capillary Electrophoresis-Mass Spectrometry (CE-MS): Useful for separating ionic compounds, particularly small polar metabolites. Offers high efficiency and resolution with minimal sample volume requirement.
- High-Resolution Nuclear Magnetic Resonance (NMR) Spectroscopy: Provides highly detailed information about the molecular structure, dynamics, and interactions. Non-destructive and does not require chromatographic separation, preserving the sample for further analysis.
- Fourier-transform infrared (FTIR) Spectroscopy and Raman Spectroscopy: Useful for molecular fingerprinting and identifying functional groups. Provides complementary information to MS and NMR data.
Technical Classifications
The methods we utilize for metabolite identification can be classified based on their analytical platforms and application purposes:
- Targeted Metabolomics: Focuses on the analysis of predefined sets of metabolites, provides precise quantitative data and is often used in validation studies.
- Untargeted Metabolomics: A comprehensive approach to profiling all measurable metabolites in a sample, facilitates the discovery of novel biomarkers and pathway analysis.
- Pathway Analysis: Integrates metabolomic data into biological pathways to understand the systemic responses, helps in identifying pathway alterations associated with diseases or environmental changes.
- Fluxomics: Studies the dynamic changes in metabolic fluxes in response to environmental conditions or genetic modifications, provides insights into the regulation of metabolic networks and biochemical pathways.
Application Areas
- Pharmaceutical and Biomedical Research: Drug development and metabolism studies, disease biomarker discovery and validation, personalized medicine and therapeutic monitoring.
- Environmental Sciences: Monitoring and analyzing pollutants and their biodegradation products, assessing the impact of environmental stressors on ecosystems, studying interactions between organisms and their environments.
- Agriculture and Food Science: Enhancing crop quality and understanding plant metabolism, food safety monitoring and the detection of contaminants, studying plant-microbe interactions and soil health.
- Industrial Biotechnology: Optimizing metabolic pathways for biofuel production, developing sustainable bioprocesses and bioproducts, environmental remediation and waste management.
Environmental Benefits
- Sustainable Agriculture: Analyzing plant metabolites to improve crop resilience and productivity, reducing the need for chemical fertilizers and pesticides through better understanding of plant metabolism.
- Eco-friendly Industrial Processes: Optimizing biotechnological processes to minimize waste and energy consumption, facilitating the production of biodegradable materials and biofuels.
Our Services
We offer a wide range of metabolite identification services tailored to meet the specific needs of our clients:
- Metabolic Pathway Analysis: Integrating metabolomic data with pathway and network analysis tools, providing insights into metabolic alterations and their biological significance.
- Custom Metabolomic Solutions: Tailored services to meet specific research or industrial needs, collaborative projects for method development and optimization.
- Data Interpretation and Consulting: Expert analysis and interpretation of metabolomic data, consultation services to assist with experimental design, data integration, and result interpretation.
Distinctive Service Features
- Comprehensive Analysis: Combining multiple analytical platforms provides a complete and accurate metabolite profile.
- Tailored Solutions: Customizable services to meet specific research and application requirements.
- Environmental Focus: Our mission-driven approach emphasizes sustainability and environmental health.
- Collaborative Approach: We partner with our clients, providing guidance and support throughout the project workflow.
At CD BioSciences, our metabolites identification of biological compounds service is designed to support scientific research and industrial applications through advanced analytical technologies and extensive expertise. From pharmaceutical research to environmental monitoring, our custom solutions and commitment to sustainability set us apart as a leader in the field of metabolomics. Please contact us for more information.
How to Place an Order
Reference
- Payal G., Sonam G., et al. "Techniques for Detection and Extraction of Metabolites" Engineering of Microbial Biosynthetic Pathways 2020, 17:33-51.
Our products and services are for research use only and cannot be used for any clinical purposes.
Metabolites Identification
