Keep in airtight containers at 10-30°C. Prepared media is kept at 15-30°C.
Marine Oxidation Fermentation Medium (MOF Medium) 
Catalog Number FM-2266
Product Name Marine Oxidation Fermentation Medium (MOF Medium)
Aspect Light yellow to pinkish purple powder
| Application | Based on the oxidative and fermentative metabolism of carbohydrates, this product allows the differentiation of marine bacteria. |
| Principle | Marine bacteria exhibit different reactions: carbohydrate-fermenting types turn both tubes (covered and uncovered) from red to yellow; carbohydrate-oxidizing types change only the uncovered tube. Non-oxidative, non-fermentative types do not change color and show an alkaline (red to deep pink) reaction in the uncovered medium. |
| pH | 8.0±0.2 |
| Colour and Clarity of Prepared Medium | Pink, clear to slightly milky gel |
| Formulation (/L) |
Tryptone 1.0 g Yeast extract 0.10 g Tris (hydroxymethyl) aminomethane 0.50 g Boric acid 0.0110 g Ammonium sulphate 0.50 g Disodium hydrogen phosphate 0.0040 g Ammonium nitrate 0.00080 g Sodium chloride 9.70 g Magnesium chloride 4.40 g Sodium sulphate 1.60 g Calcium chloride anhydrous 0.90 g Potassium chloride 0.2750 g Sodium bicarbonate 0.080 g Potassium bromide 0.040 g Strontium chloride 0.0170 g Sodium silicate 0.0020 g Sodium fluoride 0.00120 g Phenol red 0.010 g Agar 3.0 g |
| Directions | Suspend 22.14 g in 1000 ml of purified/distilled water. Heat to boiling to completely dissolve the medium. Autoclave at 15 pounds pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile glucose solution (or other carbohydrate of choice) to a final concentration of 1%. Mix well and dispense into sterile test tubes. |
Keep in airtight containers at 10-30°C. Prepared media is kept at 15-30°C.
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