Mannitol Motility Test Medium
Mannitol Motility Test Medium

Catalog Number FM-2274

Product Name Mannitol Motility Test Medium

Aspect Light yellow to pink powder

Product Information

Application The product is suitable for determining the activity of mannitol fermentation.
Principle Motile bacteria spread throughout the medium, while non-motile bacteria grow only along the inoculation line. Mannitol fermentation creates acidic conditions, changing the medium from red to yellow (phenol red as the pH indicator).
pH 7.6±0.2
Colour and Clarity of Prepared Medium Red, Clear to Slightly Milky Gel
Formulation (/L) Peptone 20.0 g
Mannitol 2.0 g
Potassium nitrate 1.0 g
Phenol red 0.040 g
Agar 3.0 g
Directions Suspend 26.04 g in 1000 ml of purified/distilled water. Heat to boiling to completely dissolve the medium. Dispense in tubes and sterilize by autoclaving at 15 pounds pressure (121°C) for 15 minutes. Allow the tube to cool in the vertical position.

Storage

Store in airtight containers at 10-30°C. Prepared media is stored at 15-30°C.

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