Store at 2-25°C in a light-proof place
Application | For isolation of Gram-negative Enterobacteriaceae fermenting lactose and non-fermenting lactose. |
Principle | Peptone, peptones provide carbon and nitrogen sources, vitamins and growth factors; bovine bile salts and crystal violet inhibit the growth of gram-positive bacteria; sodium chloride maintains a balanced osmotic pressure; agar is a coagulant for the medium; lactose is a fermentable sugar; neutral red is a pH indicator; colonies appear pink and a turbid ring of bile salt precipitation appears around the colonies as the bacteria ferment lactose to produce acid. |
Formulation (/L) |
Peptone 20.0 g Bovine bile salt 5.0 g sodium chloride 5.0 g agar 14.0 g Lactose 10.0 g Neutral red 0.03g Purified/distilled water 1000mL |
Directions | Unpacking can be used under clean environment. |
Store at 2-25°C in a light-proof place
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