Low Temperature α-amylase, 40,000 u/ml

Low Temperature α-amylase, 40,000 u/ml
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Catalog Number IE-1126

Physical Form Liquid

Color Brown

Declared Activity 40,000 u/ml

Overview

Description Low-temperature α-amylase, 40,000 u/ml is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. The product is not as heat-stable as thermostable α-amylase, bringing the benefit of easy inactivation.
Mechanism This product belongs to family of hydrolytic enzyme, attacking α-1.4 glycosidic bond in starch, glycogen, etc., producing dextrin, oligosaccharides a small amount of glucose.
Dosage Brewing industry: The recommended dosage is 0.4-0.8 L of the enzyme preparation per ton of total raw materials, added at liquefaction.
For alcohol industry: Add 0.4-1 L per ton of total raw materials with pH 5.0-6.0.
Fruit juice industry: The recommended dosage is 0.02-0.1 L/T fruit.
The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.

Dosage

Brewing industry: The recommended dosage is 0.4-0.8 L of the enzyme preparation per ton of total raw materials, added at liquefaction.
For alcohol industry: Add 0.4-1 L per ton of total raw materials with pH 5.0-6.0.
Fruit juice industry: The recommended dosage is 0.02-0.1 L/T fruit.
The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.

Specifications

Declared Activity 40,000 u/ml
Physical Form Liquid
Color Brown
Odour Normal microbial fermentation odour.
Lead ≤5.0 mg/kg
Arsenic ≤3.0 mg/kg
Total Viable Count ≤50000 CFU/ml
Coliform Bacteria ≤30 CFU/ml
Escherichia coli <10 CFU/ml, ≤3.0 MPN/ml
Salmonella/(25g) Not Detected
Bulk Density 1.05-1.20 g/ml

Reaction Parameters

Activity Temperature 40°C-85°C
Optimum Temperature 60°C-75°C
Activity pH 5.0-7.0
Optimum pH 5.0-6.0

Processing Condition

pH 3.5–5.5 (25°C)

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