Lactose Bile Medium (high pH) | Application | Determination of coliforms, fecal coliforms, and E. coli. |
| Principle | Peptones provide a carbon and nitrogen source to meet the needs of bacterial growth, bovine bile salts inhibit the growth of gram-positive bacteria, lactose is a fermentable sugar for coliforms, and bromocresol violet is a pH indicator that appears yellow in acidity and purple in alkalinity. |
| pH | 8.3±0.1 |
| Formulation (/L) |
Peptone 20.0 g Bovine bile salt 5.0 g Lactose 10.0 g Bromocresol purple 0.01 g |
| Directions | Weigh the product 35.0g, heat and dissolve in 1000ml distilled water, divided into 20mm×150mm test tubes with inverted fermentation tubes, 10ml per tube, autoclaved at 115°C for 15 minutes, spare. |
| Shelf Life | 3 years |
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