Kompetitive Allele-Specific PCR (KASP)

• SNP Selection: We identify species-specific SNPs that are diagnostic for your target species.
• KASP Assay Design: For each SNP, we design two allele-specific primers, each corresponding to one of the SNP alleles. A common reverse primer is also included. Each allele-specific primer is tagged with a unique tail sequence that is complementary to a fluorescently labeled probe.
• PCR Amplification: Your DNA samples are amplified using the KASP assays. During PCR, only the allele-specific primer that perfectly matches the SNP in the sample will bind and extend.
• Fluorescence Detection: The extended primer enables the corresponding fluorescent probe to bind, generating a specific fluorescent signal.
• Species Identification: By analyzing the combination of fluorescent signals from multiple SNPs, we can accurately identify the species present in your samples.

KASP offers several advantages over traditional species identification methods:

• High Accuracy: KASP is highly specific, enabling accurate discrimination of even closely related species.
• High Throughput: KASP assays are amenable to automation, facilitating rapid and cost-effective analysis of large sample sets.
• Cost-Effectiveness: Compared to other genotyping technologies, KASP offers an economical solution, especially for large-scale projects.
• Versatility: KASP is applicable to a wide range of species and sample types, from plants and animals to microorganisms.

• Environmental Monitoring and Conservation: Identify species composition in environmental samples to assess ecosystem health and monitor changes over time. Rapidly and accurately detect invasive species to inform management strategies. Monitor populations of endangered species to inform conservation efforts.
• Agriculture and Food Safety: Verify the identity of crops and livestock for breeding programs, traceability, and food authentication. Detect adulteration and fraud in food products by identifying species present.
• Forensic Science and Law Enforcement: Analyze DNA samples for human identification in forensic investigations. Identify species from trace evidence, such as hair, feathers, or bones, to combat wildlife crime..

• Reduced Environmental Impact: KASP requires minimal sample material and reagents, minimizing waste generation.
• Conservation Efforts: By facilitating accurate species identification, KASP aids in biodiversity conservation and the protection of endangered species.
• Sustainable Agriculture: KASP supports sustainable agriculture practices by enabling efficient breeding programs and ensuring food authenticity.

• Genomic DNA Analysis: We provide comprehensive genomic DNA analysis, identifying species-specific SNPs and InDels with high precision across a wide range of genomic DNA samples, including complex genomes.
• Custom KASP Assay Design: Our team of experts designs robust and reproducible KASP assays targeted to your SNPs of interest, utilizing customer-provided SNP sequence information.
• High-Throughput Genotyping: With our KASP technology, we can handle large sample numbers and SNP genotyping projects efficiently, making it ideal for both small-scale studies and large-scale breeding programs.
• Species Identification and Germplasm Resource Identification: We apply KASP in various plant-breeding applications, including the identification of germplasm resources and quality control analysis.