High-Temperature α-Amylase, 40,000 u/ml

High-Temperature α-Amylase, 40,000 u/ml
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Catalog Number IE-1125

Physical Form Liquid

Color Brown Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.

Declared Activity 40,000 u/ml

Overview

Dosage For beer industry: The recommended dosage is 0.2-0.5 L of the enzyme preparation per ton of total raw materials, added at liquefaction. However, the optimal dosage depends on the composition of the raw materials and the specific process parameters and should be determined by testing different dosages at the brewery.
For alcohol industry: Add this enzyme 0.3-0.6 L per ton of total raw materials with pH 5.5-6.5, keep temperature at 85-90°C for around 120 minutes.
For starch sugar industry: Add this enzyme 0.4-0.5 L per ton of dry starch after pH adjustment. The liquefaction temperature can reach to 100±5°C and then keep temperature at 95-97°C for 60-120 minutes.

Dosage

For beer industry: The recommended dosage is 0.2-0.5 L of the enzyme preparation per ton of total raw materials, added at liquefaction. However, the optimal dosage depends on the composition of the raw materials and the specific process parameters and should be determined by testing different dosages at the brewery.
For alcohol industry: Add this enzyme 0.3-0.6 L per ton of total raw materials with pH 5.5-6.5, keep temperature at 85-90°C for around 120 minutes.
For starch sugar industry: Add this enzyme 0.4-0.5 L per ton of dry starch after pH adjustment. The liquefaction temperature can reach to 100±5°C and then keep temperature at 95-97°C for 60-120 minutes.

Specifications

Production Organism Bacillus licheniformis
Production Method Produced by submerged fermentation, separation and extraction technology.
Definition of Unit 1 unit of high-temperature α-Amylase equals to the amount of enzyme which liquefies 1 mg of soluble starch to dextrin at 70°C and pH 6.0 in 1 min.
Declared Activity 40,000 u/ml
Physical Form Liquid
Color Brown Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.
Odour Normal microbial fermentation odour.
Lead ≤5.0 mg/kg
Arsenic ≤3.0 mg/kg
Total Viable Count ≤50000 CFU/ml
Coliform Bacteria ≤30 CFU/ml
Escherichia coli <10 CFU/ml, ≤3.0 MPN/ml
Salmonella/(25g) Not Detected
Enzyme Activity ≥ 40,000 u/ml

Reaction Parameters

Activity Temperature 60°C-105°C
Optimum Temperature 80°C-95°C
Activity pH 4.5-7.5
Optimum pH 5.0-6.0

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