High-Resolution Melting Analysis (HRM)

Service Overview

Our company specializes in providing comprehensive HRM services tailored to meet the diverse needs of researchers, conservationists, and industry professionals. Our state-of-the-art laboratory is equipped with advanced instrumentation and staffed by a team of highly skilled molecular biologists dedicated to delivering accurate and reliable results. We offer a comprehensive suite of HRM services, encompassing:

• DNA Extraction: High-quality DNA is extracted from the sample using optimized protocols.
• PCR Amplification: A specific DNA fragment, often a barcode region like the cytochrome c oxidase I (COI) gene, is amplified using Polymerase Chain Reaction (PCR). This generates millions of copies of the target DNA.
• High-Resolution Melting: The PCR products are subjected to a precisely controlled melting process in the presence of a fluorescent dye. As the temperature increases, the double-stranded DNA molecules dissociate into single strands. The dye binds preferentially to double-stranded DNA, and as the DNA melts, the fluorescence decreases.
• Melting Curve Analysis: The rate of fluorescence decrease is meticulously monitored and plotted as a melting curve. The shape and position of the melting curve are characteristic of the DNA sequence, enabling species discrimination.

• High Sensitivity: HRM can detect even single nucleotide polymorphisms (SNPs), enabling discrimination of closely related species.
• Fast and Cost-Effective: The technique is rapid and amenable to high-throughput analysis, making it a cost-effective solution for large-scale studies.
• Closed-Tube System: The entire process is performed in a closed tube, minimizing the risk of contamination.
• Minimal DNA Requirements: HRM can be performed with relatively small amounts of DNA, making it suitable for analyzing degraded or limited samples.

• Reducing Reliance on Traditional Methods: HRM reduces the need for time-consuming and resource-intensive traditional methods, such as morphology-based identification.
• Minimizing Environmental Impact: The technique requires minimal sample material and reagents, reducing waste generation.
• Facilitating Conservation Efforts: By providing accurate and efficient species identification, HRM supports biodiversity conservation and ecosystem management.

At our bio-environmental company, we pride ourselves on offering cutting-edge high-resolution melting analysis (HRM) services to our clients. Our services are designed to meet the highest standards of precision and reliability in genetic analysis. Here's an overview of what we provide:

• Genetic Variation Analysis: We utilize HRM to identify genetic variations within nucleic acid sequences, which is fundamental for mutation scanning, methylation analysis, and genotyping.
• Methylation-Specific HRM (MS-HRM): Our service extends to detecting methylation differences between genes, a crucial aspect of epigenetic studies.
• Mutation Screening: We screen for mutations in target genes, identifying variant samples before sequencing analysis.
• High-Throughput SNP Genotyping: Our HRM services are capable of handling large-scale SNP genotyping, offering a cost-effective alternative to traditional sequencing.
• Data Processing and Analysis: We process melt curve data to provide clear, actionable results, utilizing HRM-compatible software for data analysis.

By choosing our HRM services, clients can expect professional, reliable, and innovative solutions for their genetic analysis needs. We are committed to delivering results that empower scientific discovery and advance research, please contact us and confirm with our team fro more information.