The fresh food industry is growing rapidly, and it is continuing to face challenges while trying to uphold operational effectiveness, innovative product development, and environmental sustainability. This as well has the food sector struggling in balancing the different parts of the supply chain. At the same level, CD BioSciences acts both as a provider of new services for the food industries as well as an innovative bio-environmental company that offers novel technologies to meet these challenges head on. As a bio-secure firm, we specialize in enzyme development for multifunctional usage in food chemistry.
Enzymes, also known as biocatalysts, have the unique ability to alter food production by replacing energy-draining chemicals. Our objective is to assist manufacturers improve their process profitability, fulfill circular economy obligations, and satisfy the new brand expectations for clean and green products. Our strong backbone of over 15 years in the field of industrial enzymology enables us to offer custom-made services to customers starting from enzyme discovery, focusing on their optimization, and ending with their mass production for different food industry verticals.
Fig 1. Update application of enzyme in food processing (Wang H., 2024)
Service Overview
We provide comprehensive enzyme development solutions designed to convert microbial capability into industrial functionality. These include, but are not limited to:
- Enzyme Customization: Metagenomic and extremophilic microorganism libraries are screened by high-throughput methods
- Protein Enhancement: Improvement of thermal and pH stability, as well as substrate specificity through directed evolution and rational design
- Fermentation Improvement: Utilizing strain enhancement and bioreactor parameter modification for economical production
- Formulary Development: Creating stabilized enzyme preparations whether in liquid, granule, or immobilized form.
- Application Validation: Testing of the enzyme preparations for validation at pilot scales for the targeted food matrices, including, but not limited to: dairy, brewing, bakery, etc.
Our solutions achieve 40-70% reduction in processing energy compared to conventional methods. This is possible due to the integration of green chemistry values and artificial intelligence powered enzyme prediction systems.
Technical Principles
Our approach of methodology integrates multi-omics with cutting-edge enzyme engineering:
Structure-Function Examination
- Modeling enzyme-substrate complexes through MD simulations or QM/MM
- Active site engineering via site directed mutagenesis
Expression System Design
- Heterologous expression in microbial hosts certified as GRAS like Bacillus subtilis and Aspergillus oryzae
- Co-factor regeneration pathway engineering via gene editing methods
Biochemical Engineering of the Process
- Determining kinetic parameters under industrial conditions-Minimum concentration Km, maximum reaction rate Vmax (kcat)
- Substrate channeling for multi-enzyme cascade reactions
- Combining all these fetches us more than 90% enzyme activity maintenance under real life processing limitations: solid loads, shear stress and inhibitory substances.
Technical Features
Our enzyme preparations outperform commercial benchmarks through:
| Feature |
Performance Metrics |
| Thermal stability |
60-110°C operational range (T50 improvement ≥15°C) |
| pH tolerance |
Active across 3.0-10.5 pH (application-specific) |
| Specific activity |
2-8X enhancement vs wild-type enzymes |
| Inhibition resistance |
Maintains >80% activity with 1M NaCl/5% ethanol |
| Shelf life |
18-24 months stability at 25°C |
Technical Categories
Hydrolases
- Amylases (starch liquefaction, gluten modification)
- Proteases (meat tenderization, dairy coagulation)
- Lipases (flavor development, lipids interesterification)
Oxidoreductases
- Glucose oxidase (dough strengthening, oxygen scavenger)
- Laccases (phenolic compound removal from beverages)
Transferases
- Cyclodextrin glycosyltransferase (encapsulation matrices)
- Transglutaminase (texturization of plant protein)
Isomerases
- Glucose isomerase (production of high fructose syrup)
Lyases
- Pectin lyase (juice clarification)
Custom Blends
- Multi-enzyme systems for the conversion of lignocellulosic biomass.
Application Areas
- Bakery: Amylase combinations that enhance structure of crumb and decrease pumping time by 35%.
- Dairy: Lactase formulations that allow for natural certification of products containing 100% of lactose-free ingredients.
- Beverage: Pectinase cocktails that increase juice outputs by 22% without the application of heat.
- Oleochemicals: Lipase interesterification of fats replacing chemical catalysts used in modifying the fats.
- Functional Foods: β-Glucanase Blend for extraction of bioactive compounds from cereals.
Environmental Benefits
- Energy: Thermal input during chemical hydrolysis is reduced by 55-70%
- Emissions: For every kg of glucose syrup produced using enzymes, 2.1 kg of CO2 emissions are avoided.
- Water: 30% CIP water use reduction achieved by immobilized enzyme reactors.
- Waste: Substrate conversion is nearly complete resulting in no waste produced from starch/protein processing.
- To reduce the resource footprint, our production facilities operate on 100% renewable energy with enzyme yields of over 25g/L.
Our Services
From idea development to product launch, we offer:
- Phase 1: Gather requirements and select enzymes.
- Phase 2: Screen for microbes and perform enzyme redesign.
- Phase 3: Fermentation pilot (50-1000L).
- Phase 4: Formulation and downstream processing.
- Phase 5: Trial and process fit.
- Phase 6: Lifecycle support and tech transfer.
Company Service Advantages
- Speed: Developing products in 6 to 9 months as opposed to the industry standard of 18 months.
- Precision: Achieved 92% predictive accuracy with machine learning models estimating enzyme activity.
- Sustainability: Enzymes sourced from non-GMO, non-pathogenic strains only.
- Costs: Unproven fermentation media significantly reduces production costs by 40%.
Contact Us
While the food processors are struggling to attain decarbonization and innovation at the same time, CD BioSciences is the go to partner for emerging solutions to enzymes. We have managed to combine extensive bioprocessing knowledge with sustainable design alongside R&D focused on application and these are highly value-acretive relationships. We enable clients to practically reinvent the food manufacturing process - one enzymatic reaction at a time - by transforming biological phenomena into industrial outcomes. Reach out to our biocatalysis team for guidance in developing your custom enzyme today.
How to Place an Order
Reference
- Wang H., Sun Z.K., et al. "Update application of enzyme in food processing, preservation, and detection" Food Bioengineering 2024, 12:12015.
Our products and services are for research use only and cannot be used for any clinical purposes.
Enzyme Preparation Development
