Enzyme Engineering

Enzyme engineering services leverage the power of natural catalysts - enzymes - to break down complex waste materials into simpler, usable compounds. This process, known as enzymatic hydrolysis, involves carefully selecting and engineering enzymes with specific properties to target the desired waste components. By optimizing enzyme activity, stability, and substrate specificity, we achieve efficient and sustainable waste valorization.

• Bioprospecting: Identifying naturally occurring enzymes from microorganisms that thrive in diverse environments.
• Genetic engineering: Modifying the genetic code of enzymes to alter their properties, such as activity, stability, and substrate specificity.
• Directed evolution: Using iterative rounds of mutation and selection to create enzyme variants with improved characteristics.
• Protein engineering: Rational design of enzymes based on their 3D structure and function.

• High efficiency: Engineered enzymes exhibit superior catalytic activity, leading to faster and more complete waste conversion.
• Specificity: Enzymes selectively target specific waste components, ensuring efficient transformation into desired products.
• Mild reaction conditions: Enzymatic reactions typically occur at moderate temperatures and pH, reducing energy consumption and environmental impact.
• Biodegradability: Enzymes are natural catalysts that degrade readily in the environment, minimizing pollution.
• Versatility: Our enzyme engineering platform can be tailored to various waste streams and desired products, offering customized solutions.

• Waste management: Converting municipal solid waste, food waste, agricultural residues, and industrial byproducts into valuable products, reducing landfill burden and promoting resource recovery.
• Biofuel production: Producing biodiesel, bioethanol, and other biofuels from renewable waste streams, contributing to sustainable energy solutions.
• Animal feed production: Generating protein-rich feed supplements from food waste and other organic waste streams, enhancing the sustainability of animal agriculture.
• Fertilizer production: Recovering nutrients from organic waste streams to produce biofertilizers, promoting sustainable agriculture and reducing reliance on synthetic fertilizers.
• Biogas production: Enhancing biogas production from anaerobic digestion of organic waste by pre-treating the waste with enzymes to improve digestibility and biogas yield.

• Reduced waste: Diverting waste from landfills, minimizing pollution and greenhouse gas emissions.
• Resource recovery: Transforming waste into valuable products, promoting a circular economy and reducing reliance on virgin resources.
• Sustainable energy: Producing biofuels from renewable waste streams, contributing to cleaner energy production.
• Reduced carbon footprint: Employing environmentally friendly enzymatic processes with lower energy consumption and reduced greenhouse gas emissions compared to traditional methods.

• Enzyme Discovery and Expression: We initiate our services with the identification and expression of enzymes associated with specific microbial strains. This involves genome sequencing, transcriptional analysis, and bioinformatics-guided approaches to find enzyme sequences for expression and analysis.
• Assay Design and Activity Assessment: We design new assays to find and assess the activity of enzyme preparations or modify and adapt previously developed assays. Our goal is to identify the enzyme sequence encoding the activity of interest, typically following expression in a microbial strain such as Escherichia coli or Pichia pastoris.