Electron Microscopy

Electron Microscopy

CD BioSciences provides cutting-edge electron microscopy (EM) services tailored for bioenvironmental applications. We specialize in utilizing the power of EM to visualize and analyze biological specimens at the nanoscale, enabling precise identification and characterization of various species. Our expertise lies in delivering accurate and detailed insights into the intricate world of microorganisms, contributing to a deeper understanding of biodiversity, ecological balance, and environmental health.

Scanning electron microscopy of the pollen grains for species identificationFig 1. Scanning electron microscopy of the pollen grains for species identification (Ana C., et al., 2019)

Introduction to Electron Microscopy

Electron microscopy is a powerful imaging technique that employs a beam of electrons to illuminate a specimen, generating images with significantly higher resolution than traditional light microscopy. This superior resolution allows for the visualization of intricate details of cellular structures, organelles, and even individual molecules, providing invaluable insights into the morphology and composition of biological samples.

Technical Principles

EM operates on the principle of electron-matter interaction. A focused beam of electrons is generated and directed towards a meticulously prepared specimen. As the electrons interact with the specimen, they scatter in various directions. These scattered electrons are collected and processed to form an image or diffraction pattern, revealing the ultrastructure of the specimen.

Technical Features

  • Unparalleled Resolution: EM offers resolution exceeding that of light microscopy by several orders of magnitude, enabling visualization of nanoscale features.
  • Detailed Morphological Information: Provides comprehensive insights into the structural organization of organisms, including internal and external features.
  • Versatility: Applicable to a wide range of biological specimens, from single cells to complex tissues and even fossilized remains.
  • Elemental Analysis: Coupled with energy-dispersive X-ray spectroscopy (EDS), EM can provide information about the elemental composition of the specimen.

Technical Classification

  • Transmission Electron Microscopy (TEM): Electrons pass through an ultra-thin section of the specimen, providing detailed information about internal structures, such as organelles, membranes, and macromolecules.
  • Scanning Electron Microscopy (SEM): Electrons scan the surface of the specimen, generating a three-dimensional image that reveals surface topography, texture, and composition.

Applications Across Diverse Sectors

EM plays a crucial role in various bioenvironmental applications, including:

  • Microbial Identification and Characterization: Identifying and classifying bacteria, viruses, fungi, and protists based on their unique morphology, size, and surface features. This is essential for understanding microbial diversity, detecting pathogens, and monitoring microbial communities in various environments.
  • Environmental Monitoring and Assessment: Analyzing microorganisms in environmental samples (water, soil, air) to assess biodiversity, detect pollution levels, and monitor the impact of environmental changes on ecosystems.
  • Bioremediation Studies: Investigating the interaction of microorganisms with pollutants and their role in bioremediation processes.
  • Ecological Research: Studying the morphology and interactions of microorganisms within their natural habitats, contributing to a better understanding of ecological relationships and food webs.
  • Paleontological Studies: Examining fossilized microorganisms to understand evolutionary relationships and past environments.

Environmental Benefits

  • Facilitating accurate identification of microorganisms: This enables targeted interventions for pollution control and bioremediation strategies.
  • Monitoring microbial communities: Helps assess the health of ecosystems and detect early signs of environmental degradation.
  • Supporting research on climate change: EM studies on microorganisms can contribute to understanding the impact of climate change on biodiversity and ecosystem functioning.

Our Services

Our state-of-the-art facilities and expert team offer a comprehensive suite of services tailored to meet the diverse needs of researchers and industry professionals. Here's an overview of the services we provide:

  • High-Resolution Imaging: Utilizing advanced Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), we deliver high-resolution images that reveal the intricate details of biological samples, from cellular structures to molecular-level features.
  • Sample Preparation: Our team specializes in preparing samples for electron microscopy, including critical-point drying, metal coating, and staining, to ensure optimal imaging results.
  • Microbial Identification: We offer services for the identification of microorganisms, including bacteria, viruses, and fungi, through SEM, which is particularly useful for observing surface structures and morphology.
  • Elemental Analysis: With Energy-Dispersive X-ray Spectroscopy (EDS) integrated into our SEM systems, we can provide detailed elemental composition analysis, which is crucial for understanding the chemical nature of biological samples.
  • Cryo-Electron Microscopy: For samples that are sensitive to radiation damage or require observation in their native state, we offer cryo-electron microscopy services, which allow for the study of samples at cryogenic temperatures.
  • 3D Imaging and Tomography: Our services include 3D imaging and electron tomography, which provide a comprehensive view of the three-dimensional structure of biological samples.
  • Consultation and Training: We provide consultation services to help clients understand the best approaches for their specific needs and offer training on sample preparation and data interpretation.

Distinctive Service Features

  • Advanced Technology: We operate the latest models of TEM and SEM microscopes, ensuring the highest resolution and clarity in imaging.
  • Expertise: Our team consists of experienced scientists and technicians who are well-versed in the nuances of electron microscopy and biological sample analysis.
  • Customized Solutions: We work closely with clients to tailor our services to their specific requirements, whether it's for research, quality control, or diagnostic purposes.
  • Rapid Turnaround: We pride ourselves on providing fast and efficient service, with quick sample processing and reporting times.

Contact Us

We are dedicated to advancing the field of biological research through our electron microscopy services. Contact us today to learn more about how we can support your work with precision and professionalism.

How to Place an Order

How to place an order

Reference

  1. Ana C., Ricardo L., et al "Characteristics of the exine and aperture of pollen grains of Eriosema and Rhynchosia" Grana 2019, 58(4):292-307.

Our products and services are for research use only and cannot be used for any clinical purposes.

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