Double Lactose Bile Medium | Application | Used for the determination of E. coli, fecal coliform, and E. coli. |
| Principle | Peptones provide a carbon and nitrogen source to meet the needs of bacterial growth,Bovine bile salts inhibit the growth of gram-positive bacteria,Lactose is a fermentable sugar by coliform bacteria,Bromocresol violet is a pH indicator that is yellow in acidity and purple in alkalinity. |
| pH | 7.4±0.2 |
| Formulation (/L) |
Peptone 40.0 g Bovine bile salt 10.0 g Lactose 20.0 g Bromocresol purple 0.02g |
| Directions | Weigh the product 70.0g, heat dissolved in 1000ml distilled water, divided into large test tubes with inverted fermentation tubes, 10ml per tube, 121°C autoclave sterilization for 15 minutes, standby. |
| Shelf Life | 3 years |
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