In the pursuit of sustainable energy solutions, biofuels have emerged as a promising alternative to fossil fuels. However, the efficient and cost-effective production of biofuels remains a challenge. At CD BioSciences, we harness the power of cutting-edge gene-editing technology, CRISPR-Cpf1, to optimize biofuel feedstocks for enhanced productivity and sustainability. Our services cater to a diverse range of clients, including research institutions, biofuel companies, and plant biotechnology firms.
Fig 1. CRISPR-Cpf1 (Bernd Z., 2015)
Service Overview
We offer comprehensive CRISPR-Cpf1 gene-editing services for various biofuel feedstocks, including:
- Herbaceous Plants: Grasses like switchgrass and Miscanthus, and other fast-growing plants suitable for biofuel production.
- Woody Plants: Poplar, willow, and other trees that can be used for lignocellulosic biomass.
- Microalgae: Photosynthetic microorganisms with high lipid content, ideal for biodiesel production.
Our services encompass the entire gene-editing workflow, from target identification and guide RNA design to transformation and screening of modified organisms. We tailor our solutions to meet specific client needs, whether it's improving biomass yield, enhancing stress tolerance, or optimizing the composition of biofuel feedstocks.
Technological Principles
CRISPR-Cpf1 is a revolutionary gene-editing tool that allows precise modifications to an organism's DNA. It functions as a molecular scissor, guided by a specific RNA molecule, to target and cut DNA at precise locations. This enables the insertion, deletion, or modification of genes, leading to desired traits in the target organism.
Technical Features
- Precision: CRISPR-Cpf1 offers high specificity, minimizing off-target effects and ensuring accurate gene editing.
- Efficiency: The system is highly efficient, allowing for rapid generation of modified organisms.
- Versatility: CRISPR-Cpf1 can be used to target multiple genes simultaneously, enabling complex genetic engineering.
- Simplicity: Cpf1 requires a shorter guide RNA compared to other CRISPR systems, simplifying the design process.
Types of CRISPR-Cpf1
CRISPR-Cpf1 services can be categorized into:
- Knockout: Disrupting gene function to study gene roles or eliminate undesirable traits.
- Knock-in: Inserting new genes or modifying existing ones to introduce novel traits.
- Base editing: Precisely changing individual DNA bases without introducing double-strand breaks.
- Multiplex editing: Targeting multiple genes simultaneously for complex modifications.
Applications
- Increased Biomass Yield: Enhance the production of biomass in herbaceous and woody plants.
- Improved Lipid Content: Increase lipid accumulation in microalgae for biodiesel production.
- Reduced Lignin Content: Modify lignin biosynthesis pathways in woody plants for easier processing.
- Enhanced Stress Tolerance: Improve resistance to drought, salinity, and pests in biofuel feedstocks.
- Optimized Carbohydrate Composition: Tailor the carbohydrate profile of plants for efficient bioethanol production.
Environmental Benefits
- Reducing reliance on fossil fuels: Optimizing biofuel feedstocks for increased production and efficiency.
- Minimizing land use: Enhancing stress tolerance in biofuel crops, allowing them to grow on marginal lands.
- Decreasing water and fertilizer usage: Improving resource use efficiency in biofuel feedstocks.
- Lowering greenhouse gas emissions: Promoting the use of sustainable biofuels.
Our Services
As a bio-environmental company specializing in gene editing services, we are proud to offer a suite of CRISPR-Cpf1 services tailored to the precise genetic editing of biomass materials, including herbaceous plants, woody plants, and microalgae. Here's an overview of our services:
- Targeted Gene Editing: Utilizing the CRISPR-Cpf1 system, we provide targeted gene knockouts and knockins, enabling the modification of specific genes within the genome of various plant species.
- High-Efficiency Genome Editing: Our CRISPR-Cpf1 system boasts high editing efficiencies, comparable to those of Cas9, with a preference for causing DNA deletions, making it a powerful tool for precise fragment replacement.
- Multiplex Gene Editing: We can perform simultaneous editing of multiple genes using a single CRISPR-Cpf1 plasmid, offering high productivity in editing and regulation.
- Gene Regulation: By fusing dCpf1 (DNase-dead Cpf1) to transcriptional regulators, we can achieve programmable DNA regulation in human cells, targeting regions around transcription start sites that are inaccessible to SpCas9.
- Homology-Directed Repair (HDR) Enhancement: We use CRISPR-Cpf1 to significantly increase the efficiency of HDR in zebrafish compared to CRISPR-Cas9, which is crucial for generating knockin mutants or reporter cell lines.
- Temperature-Controlled Genome Editing: Our system allows for temperature control of Cpf1-mediated mutagenesis, providing different onset and size of mutant clones, which is particularly useful in ectothermic organisms.
- Optimized for Herbaceous and Woody Plants: The CRISPR/Cpf1 system enables gene deletion, insertion, base editing, and locus tagging in both monocot and dicot plants with fewer off-target effects.
- Editing Genetic Markers: We have optimized CRISPR-Cpf1 to edit genetic markers such as CAN1, URA3, MET2, MET6, and MET25, using a type II RNA promoter to guide Cpf1 endonuclease activity.
Company Service Advantages
- High Specificity and Low Off-Target Effects: CRISPR-Cpf1 has a low propensity for off-target effects, which is a significant advantage over Cas9, ensuring precise genome editing.
- T-Rich PAM Sequence Preference: Cpf1 relies on T-rich PAM sequences, expanding the range of targetable genes, especially in non-coding RNAs and UTRs.
- Shorter crRNA for Multiplexed Editing: The crRNA for Cpf1 is much shorter than that for Cas9, facilitating in vitro synthesis and making it more suitable for multiplexed genome editing.
- Versatility in Delivery: The smaller size of the Cpf1 gene compared to Cas9 offers more versatile opportunities, particularly when delivered using AAV-based viral vectors.
Contact Us
By leveraging the unique properties of CRISPR-Cpf1, we are committed to providing cutting-edge molecular marker services that drive innovation in the field of bio-environmental science, please contact us for more information.
How to Place an Order
Reference
- Bernd Z., Jonathan S., et al. "Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System" Cell 2015, 163(3):759-771.
Our products and services are for research use only and cannot be used for any clinical purposes.
CRISPR-Cpf1
