Chromatography

Chromatography

CD BioSciences is a leading bio-environmental company specializing in providing cutting-edge chromatography services for accurate and reliable biological species identification. Our expertise lies in utilizing advanced chromatographic techniques to analyze complex biological samples, enabling precise species determination across various applications.

Fig 1. Identification of animal species by nucleic acid chromatography of hair samples (Koichi S., et al., 2023)

Introduction

Chromatography is a fundamental analytical technique employed to separate and identify different components within a mixture. Its versatility and precision make it an indispensable tool in biological species identification, allowing us to analyze intricate biological samples such as blood, tissue, environmental samples, and more. By separating and analyzing biomolecules like DNA, proteins, and metabolites, we can identify species-specific markers and patterns, leading to accurate species identification.

Technical Principles

Chromatography operates on the principle of differential partitioning between a stationary phase and a mobile phase. The sample mixture is introduced into the mobile phase, which carries it through the stationary phase. Different components within the mixture interact differently with the stationary phase based on their physical and chemical properties. This differential interaction leads to the separation of components as they migrate through the stationary phase at varying rates.

Technical Features

Chromatography services offer numerous advantages for biological species identification:

High Sensitivity: Detect and identify even trace amounts of biomolecules, enabling species identification from minute samples.
Specificity: Target specific biomolecules like DNA or proteins for accurate species determination.
Versatility: Analyze a wide range of biological samples, including blood, tissue, environmental samples, and processed food products.
Rapid Analysis: Obtain results quickly and efficiently, facilitating timely decision-making.
Cost-Effectiveness: Offer competitive pricing and customized solutions to meet specific project needs.

Technical Classification

We utilize a variety of advanced chromatography techniques tailored to specific applications:

High-Performance Liquid Chromatography (HPLC): This versatile technique is used for separating and analyzing a broad range of biomolecules, including DNA, proteins, and metabolites. It offers high resolution and sensitivity, making it suitable for complex biological samples.
Gas Chromatography-Mass Spectrometry (GC-MS): Primarily used for analyzing volatile compounds and metabolites, GC-MS helps identify species-specific metabolic fingerprints. This technique is particularly valuable in environmental monitoring and food safety applications.
Thin-Layer Chromatography (TLC): A simple and cost-effective technique, TLC is often employed for initial screening and separation of biomolecules. It is widely used in educational settings and for preliminary analyses.

Applications Across Diverse Sectors

Environmental Monitoring and Conservation:

Identify species in environmental samples to assess biodiversity, track invasive species, and monitor endangered populations.

Analyze water and soil samples to detect pollution and identify sources of contamination.

Food Safety and Authenticity:

Verify the authenticity of food products by identifying species origin, preventing adulteration and fraud.

Detect the presence of allergens and contaminants in food, ensuring consumer safety.

Forensic Science and Wildlife Crime Investigation:

Analyze biological evidence from crime scenes to identify species involved, aiding in investigations.

Combat wildlife trafficking by identifying species from seized samples, such as animal parts or derivatives.

Agriculture and Fisheries:

Identify plant and animal pathogens to prevent disease outbreaks and protect crops and livestock.

Monitor the quality and safety of agricultural products, ensuring compliance with regulations.

Biotechnology and Pharmaceutical Research:

Analyze and purify biomolecules for research and development of pharmaceuticals and other biotechnological products.

Identify and characterize novel species with potential applications in biotechnology.

Environmental Benefits

Reduced Environmental Impact: Our techniques minimize the use of hazardous chemicals and solvents, reducing environmental impact.
Sustainable Practices: We adhere to sustainable laboratory practices, including waste reduction and responsible resource management.
Conservation Efforts: Our services support conservation efforts by providing accurate species identification for biodiversity monitoring and protection of endangered species.

Our Services

We are committed to providing cutting-edge chromatography services that cater to the complex needs of the bio-environmental industry. Our state-of-the-art facilities and highly skilled team of scientists leverage the power of chromatographic techniques to deliver precise, reliable, and actionable insights into your samples:

High-Performance Liquid Chromatography (HPLC):

Comprehensive analysis of a wide range of compounds, including peptides, proteins, and small molecules. Reversed-phase (RP-HPLC) for separation based on hydrophobic interactions. Hydrophilic interaction liquid chromatography (HILIC) for polar compound separation. Size-exclusion chromatography (SEC) for determining molecular weights of polymers and proteins.

Gas Chromatography (GC):

Analysis of volatile organic compounds (VOCs) in environmental samples. GC-Mass Spectrometry (GC-MS) for the identification and quantification of complex mixtures.

Capillary Electrophoresis (CE):

High-resolution separation of small molecules, ions, and macromolecules. Capillary Zone Electrophoresis (CZE) for the analysis of DNA, RNA, and proteins.

Supercritical Fluid Chromatography (SFC):

Environmentally friendly alternative to traditional HPLC using supercritical fluids. Ideal for the separation of thermolabile and non-polar compounds.

Countercurrent Chromatography (CCC):

A support-free liquid-liquid extraction technique for the preparative-scale separation of natural products. Thiolysis HPLC for Procyanidins Analysis: Specialized method for the analysis of procyanidins in cranberry and other berry products.

NMR and IR Spectroscopy:

Structural elucidation and confirmation of compound identity post-chromatography. Comprehensive analysis of metabolic profiles and fingerprinting of biological samples.

Distinctive Service Features

Experienced Team: Our team comprises highly skilled scientists with extensive experience in chromatography and biological species identification.
State-of-the-Art Facilities: We utilize cutting-edge equipment and technology to ensure accurate and reliable results.

Quality Assurance: We adhere to strict quality control measures to maintain the highest standards of accuracy and precision.
Customer Focus: We prioritize client satisfaction by providing personalized service and tailored solutions.

Contact Us

At CD BioSciences, we are dedicated to exceeding your expectations through our advanced chromatography services. Partner with us for reliable, innovative, and environmentally conscious solutions tailored to the bio-environmental sector. Please contact us for more information.

How to Place an Order

Reference

Koichi S., Rie I., et al "Identification of animal species by nucleic acid chromatography of hair samples and investigation of its applicability to human biological samples" Forensic Science International 2023, 351:111811.

Our products and services are for research use only and cannot be used for any clinical purposes.

Solutions

Inquiry