Cellobiase for Deglycosylation of Glycoside

Cellobiase for Deglycosylation of Glycoside
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Catalog Number IE-1215

Physical Form Liquid

Color Light to dark brown

Dosage Parameters 0.3-0.5% on basis of dry raw material

Source Microorganism

Overview

Summary It contains mainly cellobiase but also some other beneficial activities like rhamnosidase, xylanase, β-glucanase, etc. It is able to hydrolyze glycosidic linkage with β configuration to produce aglycone.
Description The product is produced through submerged fermentation of a carefully selected microbial strain, followed by separation, formulation, and drying. It primarily contains cellobiase, along with additional beneficial activities such as rhamnosidase, xylanase, β-glucanase, cellulase, and other hemicellulases.
Cellobiase efficiently hydrolyzes glycosidic linkages with β-configuration to produce aglycones. This enzyme can be used as an alternative to acid or alkali in traditional processes for the deglycosylation of glycosides.
Advantages Efficient transformation of glycoside into aglycone
Enzymatic deglycosylation
Avoid the use of acid or alkali
Application For deglycosylation of glycoside
Application Area Wine enzymes
Plant extract
Tobacco applications
Food
Application Recommendation The recommended dosage 0.3~0.5% on basis of dry raw material. The dosage has to be optimized based on raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
Before dosing soak raw material in warm water for 30min, disperse the enzyme in 40°C water and add into plant raw materials, stir gently to mix, adjust pH and temperature before addition of the enzyme when necessary to ensure required reaction condition, keep at 40°C for 24-72hrs.

Specifications

Dosage Parameters 0.3-0.5% on basis of dry raw material
Optimum Temp 45-50°C
Effective Temp 40-55°C
Optimal pH 4.5-5.0
Effective pH 4.5-6.0
Source Microorganism
Physical Form Liquid
Color Light to dark brown
Scope of Shipment Worldwide

Package and Storage

Package 10 kg/plastic barrel
Storage Keep sealed in a dry and cool place and avoid direct sunlight.

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