Bacillus thuringiensis israelensis (Bti) serves as a biological larvicide specifically targeting the larvae of mosquitoes (Culiseta longiareolate), fungus gnats (Bradysia impatiens), and black flies (Simulium vittatum Zetterstedt). When applied to aquatic environments where these pests breed, Bti effectively controls larval populations while remaining safe for beneficial insects, wildlife, humans, pets, and livestock. Bti can be used in a wide range of water-containing habitats, including both natural and artificial ponds and lakes, wastewater treatment facilities, sewage lagoons, irrigation ditches, catch basins, rice fields, and any standing water with mosquito larvae in agricultural areas.