Bacillus subtilis-SDZHYB-10 | Application | Research; Teaching, It has the effect of solving phosphorus and potassium, and the liquid fermentation solution and its products also promote the growth of crop roots, which can be used to prepare microbial fertilizer and bio-organic fertilizer. |
| Directions | 1. Prepare 1 test tube containing 5~10mL of pre-oxygenated liquid culture medium; 2. In a safety cabinet, cauterize the top of the ampoule with an alcohol lamp, rupture it by rapid dripping, and crack it with tweezers; 3. Aspirate 0.240765205mL of liquid medium into the ampoule, fully dissolve the powdered bacteria, and then aspirate it back into the test tube; 4. Place the test tube under suitable culture conditions and wait for the growth of the strain. |
| Synonyms | SDZHYB-10 |
| Note | 1. Before activation, place the freezing tube in a low temperature, dry and clean place, avoiding room temperature placement leading to the decline of the strain; 2. The operation of opening and re-solubilization should be carried out aseptically to prevent contamination and infection; 3. Only one strain is operated at a time, and when multiple strains are operated, it is necessary to disinfect the equipment, instruments, etc.; 4. If you find any abnormality such as loose cap of the freezing tube and muddy re-solubilization solution, discontinue the use of the freezing tube. |
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