Bacillus methylotrophicus is a gram-positive bacterium that was isolated from the rhizosphere of traditionally grown rice in field conditions. This strictly aerobic, motile, rod-shaped bacterium forms endospores and thrives best at 30°C and pH 7.0 when grown in ammonium mineral salts (AMS) medium with 600 mM methanol. It serves as an effective biocontrol agent against the mycelial growth and conidial germination of several plant pathogenic fungi, such as Botryosphaeria dothidea, Phyllosticta ampelicida, Valsa ceratosperma, and Botrytis cinerea. Additionally, it can enhance the growth of tomato plants in both greenhouse and field environments.