Anaerobic Fermentation Medium Base

Anaerobic Fermentation Medium Base
Anaerobic Fermentation Medium Base

Catalog Number FM-2263

Product Name Anaerobic Fermentation Medium Base

Aspect Milky White to yellow powder

Product Information

Application This product can be used for the detection of anaerobic microbial fermentation reactions.
Principle Non-sporing anaerobes are diverse opportunistic pathogens commonly found as normal flora on skin and mucosal membranes. The medium contains biopeptone and peptone B to supply nitrogen, chloride, and other vital nutrients for bacterial growth. Sodium helps maintain osmotic balance, while agar acts as a solidifying agent.
pH 7.2±0.2
Colour and Clarity of Prepared Medium Yellow, clear to slightly milky white gel
Formulation (/L) Biopeptone 16.0 g
peptone B # 4.0 g
Sodium chloride 5.0 g
Agar 15.0 g
Directions Suspend 40.0 g in 1000 ml of purified/distilled water. Heat to boiling to completely dissolve the medium. Autoclave at 15 pounds pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5- 7% sterile horse blood. Stir well before pouring.

Storage

Store in airtight containers at 10-30°C and prepared media at 2-8°C.

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