Acetolactate Synthase
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Catalog Number IE-1393

Physical Form Liquid

Color Brown to dark brown

Dosage Parameters 0.1-1 kg/T of raw material

Source Microorganism

Overview

Summary It's primarily used to catalyze the formation of acetyl lactate or 2-acetyl-2-hydroxybutyrate from pyruvic acid and acetoacetic acid or 2-ketovaleric acid.
Description Acetolactate Synthase is obtained through submerged fermentation, followed by separation, ultrafiltration, and formulation. This enzyme plays a crucial role in catalyzing the formation of acetyl lactate or 2-acetyl-2-hydroxybutyrate from pyruvic acid and acetoacetic acid or 2-ketovaleric acid. Additionally, it functions as a decarboxylase, facilitating the decarboxylation of isovaleric acid into isobutyraldehyde. Due to its enzymatic versatility, this product is primarily utilized in the pharmaceutical industry.
Mechanism Acetolactate Synthase catalyzes the decarboxylation of one molecule of pyruvic acid and its condensation with another molecule of pyruvic acid to form one molecule of acetyl lactate, thereby leading to the biosynthesis of the side-chain amino acids valine, leucine, and isoleucine.
Advantages Enzymatic synthesis of acetyl lactate; decarboxylation of isovaleric acid into isobutyraldehyde.
Application Enzymatic synthesis of acetyl lactate
Application Area Food
Biosynthesis
Application Recommendation The recommended dosage is 0.1-1 kg/T of raw material. The dosage has to be optimized based on real situation, the raw material specifications, product expectations and processing parameters. It is better to begin the test with the smallest quantity.

Specifications

Dosage Parameters 0.1-1 kg/T of raw material
Optimum Temp 35-45°C
Effective Temp 35-55°C
Optimal pH 6.0-8.0
Effective pH 4.0-9.0
Source Microorganism
Physical Form Liquid
Color Brown to dark brown
Scope of Shipment Worldwide

Package and Storage

Package 25 kg/jerry can (customizable)
Storage Keep sealed in a dry place at below -8°C and avoid direct sunlight.

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