18S Sequencing

18S sequencing exploits the evolutionary conserved nature of the 18S ribosomal RNA (rRNA) gene, a crucial component of the eukaryotic ribosome responsible for protein synthesis. This gene exhibits both highly conserved and variable regions, making it an ideal marker for phylogenetic analysis and species identification.

The process involves extracting DNA from the target organism or environmental sample, followed by PCR amplification of the 18S rRNA gene using specific primers. The amplified DNA fragments are then sequenced, and the resulting sequences are compared against extensive databases of known 18S rRNA sequences to identify the organism or determine its closest relatives.

• Universality: The 18S rRNA gene is ubiquitous in eukaryotic organisms, enabling the identification of a broad range of species, from microscopic algae to large mammals.
• Specificity: The variable regions within the 18S rRNA gene allow for differentiation between closely related species, enhancing the accuracy of taxonomic classification.
• Sensitivity: 18S sequencing can detect even minute quantities of DNA, making it suitable for analyzing complex environmental samples with low target organism abundance.
• High-throughput: Advances in sequencing technology enable the simultaneous analysis of multiple samples, increasing efficiency and reducing turnaround time.

• Biodiversity Assessments: Characterizing species richness and diversity in various ecosystems, such as forests, grasslands, and aquatic environments.
• Environmental Monitoring: Assessing the impact of pollution, climate change, and other environmental stressors on biological communities.
• Bioremediation: Monitoring the effectiveness of bioremediation strategies by tracking changes in microbial community composition.
• Invasive Species Detection: Identifying and tracking the spread of invasive species, enabling timely intervention and management.
• Ecological Studies: Investigating species interactions, food webs, and other ecological processes.

• Conservation efforts: Identifying endangered and threatened species, facilitating their protection and habitat restoration.
• Sustainable resource management: Monitoring biodiversity changes in response to resource extraction and land-use practices.
• Pollution control: Assessing the impact of pollution on aquatic and terrestrial ecosystems, guiding mitigation strategies.
• Climate change adaptation: Understanding how climate change affects species distribution and community composition, informing adaptation measures.

At our bio-environmental company, we offer a comprehensive suite of 18S rRNA sequencing services designed to accurately identify and classify eukaryotic species within complex samples. Our services include:

• Next-Generation Sequencing (NGS) and Long-Read Sequencing Platforms: We utilize both NGS and long-read sequencing technologies to identify specific 18S ribosomal RNA (rRNA) genes in eukaryotes and yeasts, providing a detailed analysis of microbial communities and evolutionary relationships.
• Full-Length 18S rRNA Sequencing: Our service offers full-length sequencing of the 18S rRNA gene, which enhances the precision of species identification and provides a more accurate reconstruction of microbial communities.
• Data Quality and Analysis: We ensure strict data quality control with a rigorous quality control system and offer a diverse range of analytical content, including OTUs clustering, species annotation, and diversity indexes generation.

Contact Us

By leveraging our advanced sequencing technologies and expertise, we are committed to providing you with the highest quality 18S rRNA sequencing services to support your research and environmental assessments. Contact us today to discuss how we can meet your specific project requirements.